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KMID : 0578319950050010030
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Molecules and Cells
1995 Volume.5 No. 1 p.30 ~ p.34
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Isolation and Nucleotide Sequence Analysis of Maize cDNA Clones Encoding Small GTP-binding Proteins
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Kang, Kwon Kyu
Nou, Ill Sup/Lee, Hyo Yeon/Lee, Seon Ha/Toshiaki, Kameya
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Abstract
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cDAN fragments encoding small GTP-binding proteins generated by polymerase chain reaction using highly consewed regions of ras-related genes were used to screen a maize cDNA library. The two cDNA clones with the longest inserts were designated as Mgpl and Mgp2, respectively. Mgp1 clone is 941 bp long with a 633 bp open reading frame. Mgp2 clone is 769 bP long containing 648 bp open reading frame with 87 bp flanking the 5´ region and 34 bp at the 3´ end. The deduced amino acid sequence of maize GTP-binding protein (Mgp)1 showed 42% homology with Zea mays yptl protein, 51% homology with Mgp2, and 61.3% homology with rice rgp1 protein. Conserved stretches in the deduced amino acid sequence of Mgp1 and Mgp2 included four regions involved in GTP-binding, an eflector region, and C-terminal cystein residues required fo. prenylation and subsequent membrane attachment Northern blot analysis demonstrated that Mgpl mRHA was expressed in roots, shoots, ears, tassels of maize with the highest level present in tassels and ears. Mgp2 mRNA was expressed at the highest level in ears and roots of maize. Southern blot analysis shows that, in addition to Mgp1 and Mgp2, several other related genes exist in the maize genome, suggesting the two genes are members of a small multigene family.
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KEYWORD
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