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KMID : 0578319950050010041
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Molecules and Cells
1995 Volume.5 No. 1 p.41 ~ p.46
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Applicability of Fluorescently-tagged short Tandem Repeats Markers Genotyping for Forensic Application
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Seon, Moon-Suk
Kang, Soon-Ja/Choi, Sang-Kyu
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Abstract
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The genetic analysis of short tandem repeat markers based on (dC-dA)n(dG-dT)n dinucleotide repeat sequences using fluorescently-tagged primers were evaluated to the suitability for DNA typing including its accuracy, precision, and sensitivity. The average accuracy and precision of band-size estimates obtained by automated real-time analysis of fluorescently amplified products showed 99.48% and 99.83%, respectively. The minimal amount of target DNA required to detect a fluorescent signal on the Genescanner for short tandem repeat markers was approximately 0.04 ng of template genomic DNA, and allelic profiles could be obtained for cases of a highly degraded state of target DNA. Therefore, these facts shows that short tandem repeat genotyping by the automated analysis of fluorescently amplified products will be advantageous for forensic application.
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