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KMID : 0578319950050020171
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Molecules and Cells
1995 Volume.5 No. 2 p.171 ~ p.175
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NTPase Activity of hepatitis C Virus NS3 Protein Expressed in Insect Cells
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Gwack, Yousang
Kim, Dong Wook/han, Jang Hyun/Choe, Joonho
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Abstract
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The NS3 protein of hepatitis C virus is known to have a serine-type proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase activity based on the presence of sequence motifs. We demonstrated that the NS3 protein of hepatitis C virus expressed in insect cells possesses an NTPase activity. Employing a baculovirus expression system, we expressed the NS3 protein and partially purified it using a metal-binding chromatography. We found that two kinds of NS3 proteins were expressed in a baculovirus expression system. The p70 protein is an expected form and the p47 protein seems to be a proteolytic cleavage form of the p70 protein. The p70 protein showed a specific NTPase activity. NTPase activity was completely inhibited by NS3 monoclonal antibodies. Our data strongly suggest that the NS3 protein of HCV expressed in insect cells contains an NTPase activity.
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