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KMID : 0578319950050020187
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Molecules and Cells
1995 Volume.5 No. 2 p.187 ~ p.190
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Nonradioisotopic Polymerase Chain Reaction-Single Strand conformation Polymorphism Analysis of the p53 Gene in Korean Breast Cancer
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Oh, sang-Jin
Moon, Hyung-Jin/Yoon, Jung-Han/chung, Sang-Young
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Abstract
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Inactivation in the p53 tumor suppressor gene through a point mutation and/or loss of heterozygosity is one of the most frequent genetic changes found in breast cancer, with an incidence reported in a range of 15 to 50%. This high rate of mutation suggests that the gene plays a central role in the development of breast cancer. Because of the widespread mutatons in the p53 Bene, the mutant p53 gene and its protein product may be potential future targets for tumor-specific diagnostic or therapeutic interventions. We have used a simplified rapid, sensitive and nonradioisotopic single-strand conformation polymorphism (SSCP) technique to screen for p53 mutations. Twenty-six primary breast tumors were examined for mutations in exons 5-8 of the p53 tumor suppressor gene by PCR-SSCP analysis. Mutations of the p53 gene were detected in 9 (35%) of the 26 patients. A total of 12 mutations in the p53 gene were demonstrated in 9 Otumors. p53 mutations in these tumors are currently under investigation by nucleotide sequencing technique.
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