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KMID : 0578319950050030209
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Molecules and Cells
1995 Volume.5 No. 3 p.209 ~ p.216
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Isolation and Characterization of the Caprine Genomic β-Lactoglobulin Gene
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Kim Jae-Man
Kim Ji-Young
Kim Kyung-Jin
Yu Myeong-Hee
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Abstract
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In order to obtain a mammary tissue-specific promoter, the genomic clones of β-lactoglobulin, one of the milk-specific proteins, were isolated from a caprine genomic library. The nucleotide sequence and structure of the caprine β-lactoglobulin gene is highly consewed among ruminant species. The functionality and mammary cell-specific expression of the caprine BLG promoter was examined in an in vitro cell culture system. Deletion analysis of the upstream regulatory sequence suggests that mammary tissue-specificity and hormonal inducibility may be mediated by a distal upstream regulatory sequence above -109 bp rather than by a proximal promoter.
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KEYWORD
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