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KMID : 0578319950050040325
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Molecules and Cells
1995 Volume.5 No. 4 p.325 ~ p.329
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Regulation of Yolk Protein 2 Gene Expression in Drosophila immigrans
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Chung, Yun Doo
Kwon, Hyeok Choon/Chung, Ki Wha/Kim, Se Jae/Choe, Oh Mok/Yong,Namkoong, Yong/Kim, Jyungjin
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Abstract
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The three yolk protein genes (yp1, yp2 and yp3),encoding the three major polypeptide precursors of egg yolk, are developmentally regulated genes. The yps are expressed in sex-, stage-, and tissue-specific manners. It was reported that different cis-acting DNA regions are required for each of these tissue specificities in Drosophila melanogaster. In this paper, the developmental profile of yp2 expression was analyzed by northern blot and immunoblot analysis in Drosophila immigrans. The developmental profiles of mRNA and protein synthesis titers were very similar to those of D. melanogaster. The mRNA was first detected at more than 1 day after eclosion and the entire expression time of D. immigrans was longer than that of D. melanogaster. Electrophoretic mobility shift assay (EMSA) was carried out to identify the candidates for trans-acting factors that regulate the ovary-specific expression of yps. It revealed that only the crude extract from ovaries of adult females, not from fat bodies, could shift the mobility of the HindIII/PstI fragment of yp2. It suggests that this binding protein(s) may be an ovary-specific trans-acting factor(s). To purify the DNA binding protein, a glass bead binding purification method was devised. Using this method, we purified a putative DNA-binding protein with an approximate molecular weight of 55-60 kDa.
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