|
KMID : 0578319950050040393
|
|
Molecules and Cells
1995 Volume.5 No. 4 p.393 ~ p.396
|
|
|
Random Amplified Polymorphic DNA Patterns within Holstein Cows and among Various Cattle Breeds
|
|
kwon, Young Min
Yim, Dae Sung/Lee, Hyun Jun/Cho, Kwang Keun/Choi, Yun Jaie/Baik, Myung Gi
|
|
|
Abstract
|
|
|
|
This study was conducted to investigate random amplified polymorphic DNA (RAPD) patterns within Holstein cows and among various cattle breeds. To find RAPD markers related to milk production trait, genomic DNA was extracted from blood samples of 21 Holstein cows with varying milk production records. The amplified DNA by PCR (polymerase chain reaction) was analysed by 1.5% agarose gel electrophoresis. The amplified DNA was 1 to 10 strands in number and 0.1~2.3 kb in size when 100 kinds of random primers were used. With 15 random primers, amplification did not occur, and with 84 random primers, banding patterns of amplified DNA were all the same among the templates. RAPD polymorphism was detected in only one random primer. But there was no significant relationship between the RAPD polymorphic bands and milk production trait. To assess RAPD patterns among cattle breeds, genomic DNA was prepared from eight cattle breeds and used as templates in PCR reactions. In the case of 7 random primers, there were no differences among breeds. Three primers produced polymorphic banding patterns among breeds, in which a potential RAPD marker specific to Korean native cattle and Holstein was detected.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|