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KMID : 0578319950050050448
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Molecules and Cells
1995 Volume.5 No. 5 p.448 ~ p.451
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Cloning of Gene for β-Glucosidase from Ruminococcus albus 7
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Woo, Jung Hee
Cho, kwang Keun/Min, Hae Ki/Choi, Yun Jauc
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Abstract
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A gene coding for β-glucosidase from the rumen bacteria, Ruminococcus albus 7, was cloned into Escherichia coli DH5α by using plasmid pUC19. A R. albus 7 genomic library was prepared in E. coli by using partially Sau3AI-digested DNA. Transformants were screened for β-glucosidase activity on the basis of formation of blue colonies on LB agar plates containing 5-bromo-4-chloro-3-indolyl-β-D-glucoside. A clone containing β-glucosidase activity was found and its recombinant DNA structure was found to contain a 3.1 kilobase insert. β-Glucosidase activity against p-nitropheny1-β-D-glucoside (PNPG) expressed by the E. coli clone was found to be 42 times greater than that expressed by the original R. albus 7.
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