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KMID : 0578319950050050501
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Molecules and Cells
1995 Volume.5 No. 5 p.501 ~ p.507
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Effects of Nerve Growth Factor, Insulin, and Extracellular Matrix Proteins on the neurite Outgrowth of SK-N-BE92) Human Neuroblastoma Cells
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Hwang, Jung-Jin
Lim, Joo-Hyun/Kwon, Jung-Hee/Lee, Kyung-Young/Hur, Kyu Chung
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Abstract
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Neurite outgrowth was measured in SK-N-BE(2) [BE(2)] cells treated with nerve growth factor (NGF), insulin, or extracellular matrix (ECM) proteins to examine neuronal differentiation of this cell line. No morphological change or neurite formation were observed in the cells treated with either NGF or insulin, while cells cultured on the either laminin- or collagen- coated plate formed neurites. Immunoblotting and immunoprecipitation experiments indicate that BE(2) cells have a focal adhesion kinase (FAK), which is activated by either collagen or laminin. To figure out why BE(2) cells did not respond to NGF or insulin, tyrosine-phosphorylated components were analyzed in the anti-Ras immunoprecipitates from NGF- or insulintreated cells. Tyrosine phosphorylation was increased slightly in a few proteins in the anti-Ras immunoprecipitates from either NGF or insulin treated BE(2) cells, while more tyrosine phosphorylated proteins and increased phosphorylation of each component was observed in the anti-Ras immunopreipitates from insulin-treatd PC12 cells. However, the existence of insulin receptor in BE(2) cells was confirmed using anti-insulin receptor immunoblotting. These results indicate that BE(2) cells have a signaling system for NGF and insulin is abnormal between receptors and Ras.
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