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KMID : 0578319950050050508
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Molecules and Cells
1995 Volume.5 No. 5 p.508 ~ p.513
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Intrafamilial Transmission of Helicobacter pylori Detected by Random Amplified Polymorphic DNA Fingerprinting
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Cho, Myung-Je
Lee, Woo-Kon/Jeon, Young-Seok/Kim, Jyung-hee/Kim, Seung-Hee/Baik, Seung-Chul/Rhee, kwang-Ho
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Abstract
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Random amplified polymorphic DNA (RAPD) fingerprinting was adapted for the epidemiological study of Helicobacter pylori. Of one hundred 10-mer primers screened, one primer (#243; 5´-GGGTGAACCG-3´)producing apparent polymorphic profiles for discriminating Helicobacter pylori isolates was selected. The genomic DNAs of 108 isolates of Helicobacter pylori were subjected to polymerase chain reaction for RAPD fingerprinting, and 4 amplified fragments (900 bp, 800 bp, 700 bp, and 600 bp) were found to be useful in determining types of RAPD fingerprinting. The 108 isolates of Helicobacter pylori were grouped into 11 types, as determined by band profiles of 4 amplified fragments. Type I was found most frequently. The RAPD type of Helicobacter pylori was not disease-specific since the types were diverse in the isolates from patients. Helicobacter pylori isolates from members of three families showed identical RAPD types, strongly suggesting intrafamilial transmission of Helicobacter pylori.
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KEYWORD
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