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KMID : 0578319950050050514
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Molecules and Cells
1995 Volume.5 No. 5 p.514 ~ p.521
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Physio-biochemical Regulation and Novel Protein Induced specifically during C-P Compound Biodegradation in Pseudomonas sp. Strain #A1
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Cho, Hong-Bum
Lee, Ki-Sung/Choi, Yong-Keel
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Abstract
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In this study, a strain of bacteria was isolated from herbicide-treated soil which efficiently utilizes the herbicide glyphosate (GPS; N-phosphonomethylglycine), one of the phosphonates (Pn), as the sole phosphorus (P) source. The degrading pathway of glyphosate in the isolate was examined by a physiological and biochemical method, and was investigated for a specific inducible protein for degrading glyphosate and other phosphonates such as methylphosphonate (MPn) and 2-aminoethylphosphonate (AEPn). The isolated GPS biodegrading bacteria from the soil were identified as Pseudomonas sp., which was also able to use C-P compounds, such as AEPn and MPn, as the sole P source. When GPS was supplied with inorganic phosphate at a low concentration, Pseudomonas sp. strain #A1 revealed a diauxic pattern in which it preferred to utilize inorganic phosphate rather than GPS as a sole P source. As a result of an analysis on the degradation products of GPS by thin layer chromatography, it showed that GPS degradation occurred via the C-P lyase pathway where the sarcosine was produced by a direct cut of C-P bond as a primary metabolite and transformed into glycine. Protein patterns were compared between the case of Pi starvation and that of some phosphonates, including GPS. As a result, two specific protein bands of molecular weight 62,000 and 132,000 were additionally detected, which seemed to be a porin protein specifically induced by the C-P compound or gene products related to the C-P lyase pathway or phosphonatase pathway.
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KEYWORD
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