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KMID : 0578319950050050522
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Molecules and Cells
1995 Volume.5 No. 5 p.522 ~ p.528
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Molecular Cloning and Sequencing of the 3´-Terminal Region of Cymbidium Mosaic Virus Genomic RNA and Structural Analysis of the 3´-Npncoding Region
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Ryu, Ki Hyun
Chung, So young/Yoon, Kyung Eun/Park, Won Mok
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Abstract
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A Korean isolate of the cymbidium mosaic virus (CymMV), denoted CymMV-K, was originally isolated from a naturally infected Dendrobium ´Spring Night´. Reverse transcription-polymerase chain reaction and conventional cDNA synthesis methods were applied to clone the 3´-terminal region of the viral RNA. The determined sequence contained a full length open reading frame coding for the viral coat protein and 3´-noncoding region. The amino acid sequence homology between CymMV-K and the Singapore isolate of CymMV was 94.1% with 13 amino acid substitutions. The 3´-noncoding region (NCR) of CymMV-K RNA contained three distinctive motifs (Ⅰ, Ⅱ, and Ⅲ) conserved among potexviruses. One pseudoknot structure was present at the 3´-proximal part of the 3´-NCR when secondary and three-dimensional folding structures were predicted.
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KEYWORD
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