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KMID : 0578319950050060544
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Molecules and Cells
1995 Volume.5 No. 6 p.544 ~ p.548
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Isolation and Characterization of the Drosophila melanogaster Gene Encoding the Vacuolar ATPase Subunit A
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Choi, Kang-Ryul
Lee, kwang-Soo/Oh, Younsang,Baek, Kwanghee/Yoon, Jaeswung
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Abstract
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A Drosophila melanogaster gene (dvha) encoding a vacuolar ATPase subunit A has been isolated and characterized. The gene encodes a protein which is 607 amino acids long. The deduced amino acid sequence of the cloned gene is 86.5 and 82.1% identical to those of Manduca sexta and bovine vacuolar ATPase subunit A, respectively. Analysis of a genomic clone revealed that there are three coding exons. RNA blot analysis detected two transcripts; a 2.9 kb transcript present in both adult heads and bodies; a 0.5 kb transcript present predominantly in adult heads.
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