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KMID : 0578319950050060549
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Molecules and Cells
1995 Volume.5 No. 6 p.549 ~ p.554
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Overpexpression of epidermal Growth Facator Receptor and c-myc during Glucicirticoid-induced Cell Proliferation in Human Papillomvirus-immortalized Human Oral Keratinocytes
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Min, Jung-Mi
Woo, Kyung Mi/Lee, Gene/Kook, Joong-Ki/Park, No-Hee/Min, Byung-Moo
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Abstract
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Primary human oral keratinocytes were previously transformed by transfection with cloned human papillomavirus type 16 (HPV-16) DNA, and a transformed cell line named human oral keratinocyte-16B (HOK-16B) was established. This line contained intact HPV-16 DNA in an integrated form, expressed viral genes, and demonstrated immortality. However, the cells proliferated only in keratinocyte growth medium (KGM) containing a low level of calcium and were not tumorigenic in nude mice. To determine the effect of glucocorticoid on cell proliferation in HPV-immortalized oral keratinocytes and to investigate the mode of cell proliferation, the HOK-16B cells were exposed to 10^(-7) M of dexamethasone for 3 or 180 days. We determined the degree of cell proliferation and expression of c-myc, c-fos, transforming growth factor-α (TGF-α), epidermal growth factor receptor (EGFR), and HPV-16 E6/E7 genes from these cells. Dexamethasone increased cell proliferation in a time-dependent manner, and enhanced the transcription of c-myc and EGFR. Furthermore, the level of HPV-16 E6/E7 transcripts in the cells treated with dexamethasone for 180 days was notably higher than that of the parental counterparts. These data indicate that overexpression of EGFR, c-myc, and HPV-16 E6/E7 genes may be, in part, responsible for dexamethasone-induced cell proliferation in HPV-16-immortalized human oral keratinocytes.
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