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KMID : 0578319950050060586
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Molecules and Cells
1995 Volume.5 No. 6 p.586 ~ p.594
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Secretion of Human Pancreatic Secretory Trypsin Inhibitor from Bacillus subtilis
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LEE SE-YONG
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Abstract
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In order to secrete the human pancreatic secretory trypsin inhibitor (hPSTI) from Bacillus subtilis, three different plasmids for the secretion of hPSTI were constructed using the B. subtilits α-amylase promoter and signal sequence. When B. subtilis LKS87 was transformed with each hPSTI secreting plasmid, biologically active hPSTI was secreted into the culture broth. The average amount of the secreted hPSTI was about 1,215 mg/L and the specific inhibitory activities of the secreted hPSTIs were very similar in all three cases. We wondered whether the signal peptide of hPSTI can function in B. subtilis. Therefore, plasmid pUA₂TIII was constructed containing the amyR₂promoter followed by hPSTI signal peptide and structural gene. However, no hPSTI activity was detected in the culture broth of the transformant harboring pUA₂TIII.
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KEYWORD
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