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KMID : 0578319950050060668
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Molecules and Cells
1995 Volume.5 No. 6 p.668 ~ p.672
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A Non-radioactive Method for Simultaneous Detection of Single-strand Conformation Polymorphisms (SSCPs) and Heteroduplexes
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Lee, Seung-Tae k
Park, Sang-Kyu/Lee, Kyoung-hwa/Holmes, Aturart A./Spitz, Richard A.
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Abstract
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We have developed a simple non-radioactive method for detecting point mutations in genomic DNA based on simultaneous detection of single-strand conformation polymorphisms (SSCPs) and heteroduplexes in polymerase chain reaction (PCR) products. This method provides reproducible, clear, and sensitive detection of single-base changes in PCR products up to 600 bp in length. This method, which utilizes 0.5×MDE as gel matrix and silver staining for detection of nucleic acids, thus provides a simple and rapid approach to mutation analysis and carrier detection of genetic disorders.
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