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KMID : 0578319960060030346
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Molecules and Cells
1996 Volume.6 No. 3 p.346 ~ p.351
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Random Amplified Polymorphic DNA and Restriction Fragment Length Polymorphisom Analysis to Differentiate Raced of the Rice Blast Fungus, Pyricularia oryzae, in Korea
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Hong, sun Min
kang, Kyu Young/Kim, Nam Soo/Kang, Woo Woong/Kim, Hee Kyu
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Abstract
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Twenty-four single conidium isolate from eight races of rice blast fungus, Pyricularia oryzae, in Korea were analyzed for variation in pathotype and DNA polymorphism. Race identification was based of the infection spectrum of eight Korean differential rice variety sets. DNA polymorphism was examined by random amplified polymorphic DNA (RAPD) and repetitive DNA sequences of Magnaporthe grisea (MGR-DNA) fingerprint analyses. Of the 75 RAPD bands amplified with 11 random primers, fourteen were present in all isolates and 37 were polymorphic. DNA polymorphism was observed among races and among isolates within races. Based on cluster analysis, all isolates except an isolate 88-62 (KJ201) were classified into two distinctive groups, KI-races and KJ-races, at a genetic similarity of 0.85. RAPD analysis of the blast fungal DNA gave enough polymorphism to differentiate KI-races and KJ-races. MGR-DNA based RFLP analysis gave overall similarity at 0.78. However, the genomic fingerprint of several isolates among KI-races had dissimilar genetic relatedness within races. RAPD polymorphism generated with 11 primers gave much more reliable phylogenetic grouping for races of the blast fungus in Korea than that of MGR-based restriction fragment length polymorphism.
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