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KMID : 0578319960060040405
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Molecules and Cells
1996 Volume.6 No. 4 p.405 ~ p.410
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Quick Screening of Phosphinothricin-resistant Transgenic Tobacco Plants using chlorophyⅡ Fluorescence
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Song, Ju-Dong
Lee, Mi-Hee/Chang, Ho-Sik/Lee, Choon-hwang
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Abstract
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The effects of a herbicide, phosphinothricin (PPT), on the photosynthetic apparatus of tobacco (Nicotiana tabacum L. cv. Xanthi) leaves were investigated using a chlorophyll fluorescence assay. Among parameters analyzed in this assay, decreases in Fm/Fo and qQ and increases in Fv and qR were observed in wild-type leaves incubated in the presence of 100 mg/L PPT in the light (30 μmol.m^(-2).s^(-1)). Fm/Fo was chosen as a sensitive parameter for screening, and the value was reduced in half within 12 h after the treatment of PPT. Because the rapid decrease in Fm/Fo was presumed to be due to glyoxylate accumulation rather than the accumulation of ammonia, the PPT toxicity was monitored under conditions favorable for photorespiration. In the high light (30 μmol.m^(-2).s^(-1)), a significant decrease in Fm/Fo was observed within 3 h after the PPT treatment. By elevating the temperature from 26℃ to 36℃, an increase in PPT toxicity was also observed. In tobacco plants transformed with the bar gene coding for the PPT-acetyl transferase (PAT), no significant changes in Fm/Fo were observed, and all the screened transgenic plants showed PAT activities. These results suggest that bar gene-containing plants can be screened very quickly by employing the chlorophyll fluorescence assay under photorespiratory conditions.
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