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KMID : 0578319960060040416
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Molecules and Cells
1996 Volume.6 No. 4 p.416 ~ p.421
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Expression and Processing of the Ovarian Carcinoma Marker Folate Receptor Gene in Human MCF-7 Breast Cancer Cells
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Kim Chong-Ho
Park Young-Soon
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Abstract
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The human folate receptor (hFR) is a plasma membrane protein that is anchored to the membrane via a glycosylphosphatidylinositol (GPI) tail. To investigate the function, expression, and processing of hFR in human MCF-7 breast cancer cells which do not express detectable levels of hFR, we isolated and characterized stably transfected MCF-7 clones with cDNA for hFR from human KB epidermoid carcinomas. The resulting transfectants expressed high levels of hFR and grew more rapidly than the host or mock transfected cells in media containing physiological concentrations of folates. The hFR expressed by these cells was capable of binding with folic acid and internalizing the ligand. Furthermore, the hFR was found to be correctly processed, sorted and anchored via a GPI tail to the plasma membrane of MCF-7 cells. These results indicate that hFR expressed in MCF-7 cells are functional as in the cells that normally express the receptor protein.
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