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KMID : 0578319960060040422
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Molecules and Cells
1996 Volume.6 No. 4 p.422 ~ p.428
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Molecular Cloning and Induction of β-1,3-Glucanase Gene from Nicotiana glutinosa L.
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Yun, hae Keun
Yi, So Young/Kim, Sung Uk/Son, kwang Hee/yu, Seung Hun/Bok Song Hae/Choi, Doil
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Abstract
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Cloning and characterization of disease-response genes in plants could be an initial step toward understanding the complex disease resistance mechanism. To better understand the complex step, we isolated one of the pathogenesis-related proteins, B-1,3-glucanase, cDNA from a cDNA library of Nicotiana glutinosa showing systemic resistance. One clone (GN-3) was a partial cDNA of β-1,3-glucanase 800 bp in size with a 171 amino acid coding region. This clone had a 90% nucleotide homology with the β-1,3-glucanase gene of N. tabacum cv. BY4. A deduced amino acid sequence of GN-3 clones indicated a 91% identity with the β-1,3-glucanase of tobacco, 58% with that of Lycopersicon esculentum, and 51% with that of Glycine max. Northern blot analysis showed that expression of β-1,3-glucanase mRNAs was induced by TMV infection and salicylic acid treatment. In addition to that this gene was highly induced by CuSO4 and β-aminobutyric acid which are known as inducers of plant disease resistance. The possible role of this gene expression in relation to chemical-induced plant defense responses is discussed.
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