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KMID : 0578319960060040429
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Molecules and Cells
1996 Volume.6 No. 4 p.429 ~ p.435
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an Arabidopsis cDNA Induces Under Cadmium Stress Is Homologus to Sequences Present in the Plant´s DNA Binding Proteins
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Choi, Soon-Young
Baek, Eun Mi/Lee, Se yong
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Abstract
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Cadmium is a major heavy metal that is known to have detrimental effects on plant growth and metabolism. To identify genes induced by cadmium stress, differences in gene expression between cadmium-treated and control Arabidopsis were investigated using asymmetric PCR-based subtractive hybridization. From this subtracted library full length 1.1 kb AS8 and 1.0 kb AS30 cDNAs of cadmium-induced mRNAs were isolated. Northern blot analysis showed a 3- and 5-fold increased expression in cadmium-treated cells, respectively. Especially, the expression of AS30 gene increased and decreased in 48 h cycles like circadian-controlled genes. Sequence analysis of AS30 cDNA indicates the presence of a 60 -amino acid motif found in a family of DNA binding proteins containing the AP 2(APETALA2) domain. The deduced protein sequence of AS30 was also determined to have a putative nuclear localization signal sequence and an acidic transactivation domain, suggesting that AS30 may be a kind of plant regulatory protein that could play a role in Arabidopsis heavy metal-involved metabolism.
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