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KMID : 0578319960060040456
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Molecules and Cells
1996 Volume.6 No. 4 p.456 ~ p.462
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Quantivative Autoradiography of Beta-1 and Beta-2 Adrenergic Receptor and Cellular Localization of mRNA for Beta-1 Adrenergic Receptor in Rat Brain
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Park, hae-young
Park, Kyeong-Han/Kim, Yong-Sik/Shin, Sang-Goo/Chung, Myung-Hee/Park, chan-Woong
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Abstract
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We have used both quantitative autoradiography to localize in rat brain beta-1 and beta-2 adrenergic receptors and in situ hybridization to detect expression of beta-1 adrenergic receptor mRNA in rat brain. The receptors were labeled in vitro with ³H-dihydroalprenolol (³H-DHA) that binds nonselectively to both beta-1 and beta-2 receptor subtypes. Selective inhibition of ³H-DHA binding with specific antagonists of beta-1 and beta-2 receptors allowed the visualization of beta-adrenergic receptor subtypes. High levels of beta-1 receptors were observed in the cingulate cortex, layers I-III of the cerebral cortex, hippocampus, and thalamus. Oligonucleotide labeled with ^(35)S-dATP was used for in situ hybridization. High levels of beta-2 receptors were found in the cerebellum. Approximately equal levels of beta-1 and beta-2 receptors occurred in the midbrain including substantia nigra. The hybridization signal for beta-1 mRNA was strongest in cingulate and piriform cortex, thalamic nuclei and light or moderate in somatosensory cortex and hippocampus. The pronounced differences in the ratio of beta-1 to beta-2 receptors among brain regions suggests that the subtypes of beta-adrenergic receptors may play different roles in neuronal function.
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