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KMID : 0578319960060040481
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Molecules and Cells
1996 Volume.6 No. 4 p.481 ~ p.484
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Yeast rDNA Enhancer Requires the upstream Domain of Gene Promoter to Achieve cis-Activating Effect
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Jin, yong Hwan
Joo, jae Hoon/Choe, Soo Young/Park, Sang Dai
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Abstract
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The transcription of 35 S ribosomal RNA (rRNA) is influenced by enhancer and promoter. We previously reported that an EcoRI-HpaI fragment of Saccharomyces cerevisiae ribosomal gene spacer can enhance transcription by an adjacent RNA polymerase I promoter [Schultz, M. C., Choe, S. Y., and Reeder, R H. (1993) Mol. Cell. Biol. 13, 2644-2654] in cis position. Here we present the in vitro data to elucidate the action mechanism between promoter and enhancer elements in yeast. In general, many promoter binding transcription factors were known to bind to enhancer DNA, so the transcription activity is decreased when the transcription factors bind to enhancer DNA in trans before they form a transcription complex on the promoter of the target gene. The yeast rRNA enhancer DNA in this report, however, did not decrease the transcription activity of the gene promoter when located in trans with promoter. This strongly suggests that there are no common protein factors which can bind to both enhancer and promoter DNA. On the other hand, the cis-activating effect of the rRNA enhancer disappeared when the upstream promoter domain was deleted, suggesting that pol I enhancer requires the upstream promoter domain for its enhancing activity. These results strongly suggest that the cooperative regulations between pol I enhancer and promoter elements are mediated by distinct protein factor(s) which bind either the enhancer or upstream promoter element.
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