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KMID : 0578319960060040504
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Molecules and Cells
1996 Volume.6 No. 4 p.504 ~ p.507
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Isolation of hrp2+ Gene, a new member of SNF2/SWI2 Familiy from fission Yeast Schizosaccharomuces pombe
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Jin, Yong hwan
Jangm Yeun Kyu/Kim, Min Ji/Koh, Jin Bog/Park, Jong Kun/Choi, In Soon/Park, Sang Dai
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Abstract
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The SNF2 family includes proteins from a variety of species with roles in cellular processes such as transcriptional regulation, recombination and various types of DNA repair. Several proteins with unknown function are also included in this family. In addition, the number of genes that comprises this family is rapidly expanding. Here, we report the partial cloning and characterization of hrp2^(+) gene (helicase related gene from S. pombe) which was isolated by PCR amplification using the conserved domain of SNF2 motifs. Sequence analysis of the PCR product (438-bp) showed striking evolutionary conservation among the SNF2 family of proteins. Moreover, it has been shown through the amino acids sequence alignment that Hrp2 protein is most similar to MOT1 protein. Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as hrp2^(+) gene and this gene exists as a single copy in S. pombe genome. Two transcripts of 6.7- and 3.4- kb were detected by Northern blot analysis. Furthermore, the intensities of these two bands were increased by ultraviolet (UV) irradiation. These results indicate that hrp2^(+) is a novel member of the SNF2 family of proteins and is one of the UV-inducible genes in S. pombe.
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