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KMID : 0578319960060050528
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Molecules and Cells
1996 Volume.6 No. 5 p.528 ~ p.533
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Quantitation of Ca2+ Release Channel mRNA in Pressure-overload Induced hypertrophic Rat heart by competitive Reverse Transcription and Competitive Polymerase Chain Reaction
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Michaels, Katerina
cho, Chung Ho/Moraru, Ion/Spoerel, Nikolaus/Kim, Do Han
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Abstract
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The density of calcium release channels (CRC) in pressure-overload induced hypertrophic rat hearts is significantly lower than that in sham-operated control animals (Kim et al., 1994). The present study was undertaken to assess the expression levels of CRC in shamoperated and hypertrophied animals at the transcriptional level by competitive reverse transcription and competitive polymerase chain reaction (cRT-cPCR). Severe left ventricular hypertrophy (>70%) was induced by banding the abdominal aorta. To estimate the copy number of CRC mRNA by cRT-cPCR, CRC mRNA templates of sham-operated and hypertrophied left ventricles were amplified with varying amounts of CRC competitor RNA. As a control, glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA levels were determined similarly. Our results show that CRC copy number was significantly decreased by pressure-overload induced hypertrophy (1.15±0.22×10^(10) vs. 2.03±0.20×10^(10) /μg mRNA, p<0.05), whereas GAPDH copy numbers were similar in sham-operated and hypertrophied animals. These results suggest that CRC expression is specifically downregulated at the transcriptional level during the development of left ventricular hypertrophy by pressure-overload.
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