|
KMID : 0578319960060050571
|
|
Molecules and Cells
1996 Volume.6 No. 5 p.571 ~ p.576
|
|
|
A New Trypsin Inhibitor from Hirudo nipponia
|
|
Kim, young Hye
Hong, Seok Jin/Kim, Dong Ryoung/Kim, Seung Il/Ha, Kwon-Soo/Kang, Ke Won
|
|
|
Abstract
|
|
|
|
A new trypsin inhibitor, bdellin for Korean leech (bdellin-KL), was isolated from a blood sucking leech, Hirudo nipponia. It has a molecular mass of 16156.4 Da and pI value of 4.0. The inhibitor was stable at a range of pH 1.3 through 10.0 and temperatures ranging from 20 ℃ to 90 ℃. The bdellin-KL showed inhibition constants of 1.67 ± 0.73 × 10^(-9) M for trypsin and 1.76 ± 1.29 × 10^(-9) M for plasmin but a poor activity against chymotrypsin. Over 60 amino acid residues of the N-terminal region showed a high similarity with those of the high-molecular mass bdellin B-3 (HMB), a European leech-derived trypsin-plasmin inhibitor. The first 10 residues that contained a P1 reactive site of Lys and the spacing of six cysteines in the peptide are well conserved for the two phylogenetically different species.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|