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KMID : 0578319960060050609
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Molecules and Cells
1996 Volume.6 No. 5 p.609 ~ p.614
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Purification and Characterization of Two Lysozymes from Larval Haemolymph of Cabbage Butterfly, Artogei rapae
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Yoe, Sung Moon
Bang, In Seok/Kang, Chang Soo/Kim, Hee Heoung
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Abstract
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Lysozyme is one of the antibacterial peptides that are produced by the cabbage butterfly, Artogeia rapae. Two lysozymes, lysozymes I (LI) and II (LII), have been purified by heat treatment, cation exchange, and reversed-phase chromatography from the haemolymph of immunized last instar larvae. The haemolymph immunized with sub-lethal doses of the insect nonpathogen, Escherichia coli K-12 was collected 2 days after the injection. These enzymes showed somewhat different electrophoretic mobilities in acidic PAGE, although similar mobilities were shown in SDS-PAGE. LI and LII have strong bacteriolytic activities regardless of heat treatment (100 ℃, 60 min). Molecular weights were determined to be 13, 643 for LI and 13,629 for LII by MALDI-TOF mass spectrometer. In comparing the number of amino acids and molecular weights, we suggest that Artogeia Iysozymes are similar to vertebrate type c lysozyme, although LI and LII show considerable differences in amino acid compositions.
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