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KMID : 0578319960060050615
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Molecules and Cells
1996 Volume.6 No. 5 p.615 ~ p.621
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Translational Control of the SPP2 Gene Expression in Saccharomyces serevisiae
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Choi, Hyunsic
Park, Kwanghark/Kim, Donghwa/Lim, Chang-Jin/Kim, Kyunghoon
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Abstract
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There are four AUG codons in two short open reading frames within 54 bases upstream of the initiation codon of the SPP2 gene, which is involved in pre-mRNA splicing of the budding yeast Saccharomyces cerevisiae. Fifteen different combinations of the four AUG codons were mutated to other codons. Then, SPP2-lacZ gene fusions retaining a various number of the AUGs were constructed, and the activities of β-galactosidase expressed from the fused genes were measured in yeast. The β-galactosidase activities from the mutated fusions were higher than that from the wild type fusion: the more mutated AUG codons, the higher the enzyme activities. This result strongly suggests that the AUG codons present on the 5´ leader region of the SPP2 transcript are cis-acting regulatory elements for SPP2 gene expression on a translational level.
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KEYWORD
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