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KMID : 0578319960060060666
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Molecules and Cells
1996 Volume.6 No. 6 p.666 ~ p.672
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Isolation of Anther-preferrentially Expressed Genes from Chinese Cabbage (Brassica campestris L. ssp. Pekinensis)
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Kim, Hyun Uk
Park, Seom Seok/Chung, Tae Young/Kang, Seok Kwon
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Abstract
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Dot-differential hyvridization and cDNA Southern blot analyses were successfully employed for isolating anther-preferential genes from Chinese cabbage (Brassica campestris L.ssp.pekinensis). A cDNA library was constructed using poly(A)^(+) RNAs isolated from developing anthers when the size of flower bud was from 2 ㎜ 5 ㎜ in length. Forty three anther-preferential cDAN clones out of 230 randomly chosen cDNA clones were isolated by dot-differential hybridization using anther and leaf cDNAs labeled with [α-^(32)p] dATP as probes. Using cDNA Southern blot analysis, another 5 anther-preferential cDAN clones were isolated from 14 clones which were not identified by dot-differential hybridization. Among 48 anther-preferential cDAN clones isolated, 14 clones showed sequence homology with anther- or pollen-specific genes of other plants. Ten randomly chosen anther-preferential clones were investigated for their temporal expression during anther development. The results showed that most of the anther-preferential transcripts examined began to accumulate at the early stage of microspore development and continuously increased to the late stage of anther development.
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KEYWORD
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