|
KMID : 0578319960060060697
|
|
Molecules and Cells
1996 Volume.6 No. 6 p.697 ~ p.703
|
|
|
Isolation and Characterization of Three Homeodomain-Leucine Zipper Genes, Phz1, Phz2, and Phz4 from Pimpinella brachycarpa Shoot Tips
|
|
Moon, Yong-Hwan
Choi, Sang-Bong/Kim, Jong-Il/Kim, Joon-Chul/Han, Tae-Jin/Cho, Sung-Ho/Lee, Kwang-Woong
|
|
|
Abstract
|
|
|
|
By screening a Pimpinella brachycarpa shoot tip cDNA library with the soybean homeodomain-leucine zipper (HD-Zip) gene, Gmh1, as a probe, we isolated three cDNA clones, Phz1, Phz2, and, Phz4, encoding polypeptides that contain a homeodomain and a closely linked leucine zipper motif, In both nucleotide and deduced amino acid sequences, Phz1, Phz2, and, Phz4 were highly homologous to two Arabidopsis thaliana HD-Zip genes, a soybean HD-Zip gene and a tomato HD-Zip gene in an HD-Zip motif. Genomic Southern blot analysis indicated that phz2 and Phz4 are members of a multigene family. RNase protection assay revealed that phz2 and Phz4 are expressed at all organs including leaves, petioles, roots and shoot tips. So far, these HD-Zip proteins have been found only in dicotyledonous plants. Thus, it may be speculated that Phz1, Phz2, and, Phz4 proteins could impart functional characteu unique to the dicotyledonous plants that would express them in processes common to those plants.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|