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KMID : 0578319960060060731
Molecules and Cells
1996 Volume.6 No. 6 p.731 ~ p.736
Chemical Composition and Structure of Hyphal Wall of Null-pigment Mutant of Aspergillus nudulans
Chung, Yoon Shin
chae, Keong-Sang/Han, Dong Min/Jahng, Kwang-Yeop
Abstract
A null-pigment mutant, which never forms any colored cell types, was isolated previously from Aspergillus nidulans. In this work, the mutant was analyzed for cell wall composition. The mutant was hypersensitive to Novozym234^(TM). More protoplasts were generated from the mutant more rapidly than from the wild type. Melanin was completely absent in the hyphal wall of the mutant. Carbohydrate content in the mutant hyphal wall fraction was higher and protein content was lower than in the wild type one. The ratio of alkaline-soluble fraction was reduced in the mutant hyphal wall. These phenotypes indicated that the outer layer of hyphal wall was significantly affected by the mutation. Water-soluble polysaccharides were not produced by the mutant during mycelial growHL although the normal levels of Iytic enzymes were bound to the mutant hyphal wall. These results suggested that the npgA gene, a wild type allele of the mutant gene, is necessary for modification of apical wall structures and eventually for the formation of outer layer of hyphal wall.
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