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KMID : 0578319970070020178
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Molecules and Cells
1997 Volume.7 No. 2 p.178 ~ p.186
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Effect of a Novel saponim Adjvant Derived from Quillaja Saponaria on the Immune Response to Recombinant Hepatitis B Surface Antigen
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So, Hong-Soeb
yoon, Hye-Sung/Choi, Deong-Young/Kwon, Young-Sun/sung, Jun-Ho/lee, Tae Gyu/Park, Eun-Suk
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Abstract
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Adjuvant activity of saponins extracted from the South American tree Quillaja saponaria has been demonstrated with many antigens. Recently, four saponin fractions (designated as QS-7, QS-17, QS-18, and QS-21) with adjuvant activity were purified by reverse phase chromatography. In particular, efficay of the less toxic QS-21 fraction has been demonstrated with several recombinant viral antigens including HIV gp120. Here, we report a novel saponin fraction (designated as QS-L1) derived from Quillaja saponaria. Unlike previously identified saponins, QS-L1 had a different chemical structure and showed adjuvant activity only when administered in the presence of alum-precipitated antigen. Interestingly, the QS-L1 greatly increased not only a humoral immune reponse but also cellular immune response to recombinant hepatitis B virus surface antigen (HBsAg). Furthermore, QS-L1 showed lower toxicity in vivo and in vitro than the previously identified saponin fraction, QS-21. Finally, we examined the chemical structure of the QS-L1 using mass spectroscopic analysis, carbohydrate composition analysis and NMR spectroscopic analysis. Thus our results indicated that this novel QS-L1 saponin fraction had several desirable properties required for an effective adjuvant.
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