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KMID : 0578319970070020200
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Molecules and Cells
1997 Volume.7 No. 2 p.200 ~ p.203
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Chromosomal Localization and Neural distribution of Voltage Dependent Clacium Channel β₃Subunit Gene
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Park, sun-hwa
Suh,Young-suk/Kim, Hyun/Rhyu, Im-Joo/Kim, Hyung-Lae
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Abstract
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Voltage dependent calcium channel (VDCC) mediates the influx of free calcium ions that acts as a signal transducer. The beta 3 subunit of the VDCC regulates the activation (opening) and inactivation (closing) kinetics through phosphorylation/dephosphorylation. We isolated a genomic clone of the human VDCC β_(3) subunit from a human genomic DNA library using VDCC β_(3) cDNA as a probe. We localized VDCC β_(3) with this genomic DNA on the chromosome by fluorscent in situ hybridization, and the distribution of VDCC β_(3) in the nervous system was investigated in rats by in situ hybridization histochemistry with rat VDCC β_(3) cDNA. The gene for the VDCC β_(3) was specifically localized on human chromosome 12q13. The mRNA for the VDCC β_(3) was predominently expressed in the nervous system. In the brain, a strong expression of VDCC β_(3) mRNA was found in the medial habenular nucleus, a high level of expression was observed in the olfactory bulb and cerebellum, and a relatively high level of VDCC β_(3) mRNA was localized in the cerebral cortex, caudate-putamen and hippocampal formation. Interestingly, this distribution pattern is very similar to that of the rbE-Ⅱ, mid-low VDCC 1 subunit, and it is suspected that VDCC β_(3) and rbE-Ⅱ may function together as a pair.
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