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KMID : 0578319970070020278
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Molecules and Cells
1997 Volume.7 No. 2 p.278 ~ p.283
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Isolation and characterization of a Processed Pseudo for Murine Cyclin D3
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Jun, Do Youn
kang, Hong Soon/Seu, Jung hwn/Kim, Young Ho
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Abstract
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By using radiolabeled murine cyclin D3 cDNA as a probe, two cyclin D3 genomic clon?? MCD3P-117 MCD3P-327, were isolated from a murine genomic library construc?? with murine liver DNA. Physical mapping and DNA sequence analysis revealed that the??? clones contain approximately 1.5kb uninterrupted linear sequence similar to murine ???lin D3 cDNA at the nucleotide level, the pseudogene contained 229 bp of 5´-and 371 bp ?? 3´-untranslated regions, and a recognizable complete coding region that is 90% identic?? to murine cyclin D3. This sequence is bounded by the repeat sequence (Gc/A GCTCTCC?? which is common to many processed pseudogenes. However, multiple genetic lesion?? including substitution, deletion and/or insertion events that result in modification of ??? reading frame were found in the pseudogene sequence. The pseudogene appeared ?? accumulated 67 random point mutations in the functioal coding region composed of 87?? nucleotide positions. It is thus estimated that the cyclin D3 pseudogene aro??? approximately 11 million years (Myr) ago. These data provide the first characterization ??? murine cyclin D3 pseudogene and insight into its evolutionary age.
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KEYWORD
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