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KMID : 0578319970070020284
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Molecules and Cells
1997 Volume.7 No. 2 p.284 ~ p.289
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Clonig of an E. coli RecA and Yeast RAD51 Homolog, radA,an Allele of the uvsC in Aspergillus nifulans and Its Mutator Effects
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Seong, Kye Yong
Chea, Suhn Kee/Kang, Hyen Sam
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Abstract
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We isolated a citrate synthase gene (citA) from Aspergillus nidulans. By analysis of the protein coding region, citA was shown to encode a citrate synthase (CitA) of 52.2 kDa consisting of 474 amino acid residues that were interrupted by seven introns. Also, the pre-cursor CRA protein was revealed to have an N-terminal mitochondria) targeting signal of 35 amino acid residues containing an R-3 cleavage motif, RZ,-C-Y 1 SS which supports the fact that cit4 encodes the mitochondrial form of citrate synthase of A. nidulans. South-ern blot analysis showed that citA is present as a single copy in the genome.
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