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KMID : 0578319970070030408
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Molecules and Cells
1997 Volume.7 No. 3 p.408 ~ p.413
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Purification and antifungal Activity of a Basic 34 kDa β-1,3-Glucanase from Soybean Hypocotyls Incoculated with Phytophthora sojae f. sp. Glycines
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Yi Seung-Youn
Hwang Byung-Kook
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Abstract
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Inoculation of soybean (Glycine max L. cv. Jangyup) hypocotyls with Phytophthora sojae f. sp. glycines results in a marked accumulation of some pathogenesis-related (PR) proteins. A basic β-1,3-glucanase (34 kDa) was purified from soybean hypocotyls infected by and incompatible race of P. sojae f. sp. glycines using CM-cellulose cation exchange chromatography and Bio-gel P-60 gel filtration. The purified soybean β-1,3-glucanase cross-reacted with polyclonal antibody raised against a tomato β-1,3-glucanase. The activity of β-1,3-glucanase was much higher in the infected soybean hypocotyls than the healthy ones. The β-1,3-glucanase purified from soybean inhibited spore germination and hyphal growth of the chitin-negative fungus P. sojae f. sp. glycines, but did not show any antifungal activity against the chitin-containing fungi Alternaria mali, Colletotrichum gloeosporiodes, and Magnaporthe grisea.
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