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KMID : 0578319970070030425
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Molecules and Cells
1997 Volume.7 No. 3 p.425 ~ p.430
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Endogenous Glutamate Levels Regulate Nerve Growth Factor mRNA Expression in the Rat Dentate Gyrus
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Gwag, Byung Joo
Sessler, Francis M./Ronine, Veronique/Springer, Joe E.
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Abstract
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The levels of nerve growth factor (NGF) mRNA can be regulated in vitro and in vivo in the hippocampal formation by events associated with pharmacological activation of glutamate receptors. In the present study, the level of NGF mRNA in the hippocampal formation was examined following an intrahippocampal injection of 1nmole fluorocitrate, which temporarily inhibits the astrocyte metabolic activity in vivo. consistent with previous findings, fluorocitrate treatment significantly increased glutamate levels and decreased glutamine levels in the denate gyrus as determined by in vivo microdialysis. The increased ratio of glutamate to glutamine was followed by a significant increase in NGF mRNA expression selectively in dentate gyrus granule cells. The effects of increasing glutamate levels were blocked by pretreatment with 50nmole 2-amino-5-phosphonovalerate (AP5), a competitive antagonist that acts at the N-methyl-D-aspartate (NMDA) glutamate receptor subtype. These findings suggest that NGF mRNA expression is regulated, in part, by changes in endogenous glutamate levels, partially through enhanced excitatory neurotransmission through NMDA receptors.
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