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KMID : 0578319970070040478
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Molecules and Cells
1997 Volume.7 No. 4 p.478 ~ p.481
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Km genotyping Using Polymerase Chain Reaction-Allel Specific Oligonucleotide in Koreans
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Park, Kyung Sook
Nam, jung Hyun/Kim, Min Young
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Abstract
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The allotype of human immunoglobulin kappa light chain constant region gene (IGKC) has been identified by the serological assay or DNA typing method. The three allotypes Km*1, Km*1,2 and Km*3 have amino acid substitutions at positions of codons 153 and 191 in the kappa light chain constant region. The Km allotypes were designated as follows: Km*1 has valine (GTC) at position 153 and leucine (CTC) at 191; Km*1,2 has Ala^(153)(GCC) and Leu^(191)(CTC); Km*3 has Ala^(153)(GCC) and Val^(191)(GTC). In this study, polymerase chain reaction-allele specific oligonucleotide (PCR-ASO) was used to provide a specific allelic typing system for the IGKC gene in 213 unrelated Koreans. For Km genotyping, the IGKC coding regions plus 35 bp of the 3´ untranslated regions were amplified with specific primiers. Amplified genes were hybridized with Km ASO probes at positions of codons 153 and 191 and were detected using a colorimetric detection method. The frequencies of Km*1,2/Km*1,2, Km*1,2/Km*3, and Km*3/Km*3 were 17.8%(n=38), 51.7%(n=110), and 30.5% (n=65), respectively. The estimated allele frequencies for Km*1,2 and Km*3 were 0.44 and 0.56, respectively. The homozygote or the heterozygote of Km*1 was not observed in this study. The PCR-ASO methos distinguished the alleles Km*3, Km*1,2, and Km*1 and also distinguished the homozygote from heterozygote. Km frequencies vary significantly among various populations. Km*3 in Koreans was very similar to those observed in northern Chinese, Japanese, and the native people of British Columbia. It was lower than that reported in Caucasian, Indian, or southern Chinese.
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