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KMID : 0578319970070040509
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Molecules and Cells
1997 Volume.7 No. 4 p.509 ~ p.513
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Hinnavin I, an antibacterial peptide from Cabbage Butterfly, Artogeia repae
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Bang, In Seok
Son, Seung yeol/Yeo, Sung Moon
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Abstract
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We have previously isolated four antibacterial peptides from the immune haemolymph of the fifth instar larvae of cabbage butterfly, artogeia rapae [Yoe, S.M., Bang, I.S., Kang, C.S., and Kim, H.J. (1996) Mol. Cells 6, 609-614]. They were induced by live, nonpathogenic Gram negative bacteria. One of these novel anatibacterial peptides was named hinnavinⅠ. HinnavinⅠ is heat stable; its activity was retained after 60 min incubation at 100℃, being effective against Gram negative and/or Gram positive bacteria. HinnavinⅠ and lysozyme Ⅱ showed a powerful synergistic effect on the inhibition of bacterial growth. Amino acid composition was analyzed and the molecular weight was determined to be 4,139.94±10.91 Da by the ESI mass spectrometer. To elucidate the primary structure of hannavinⅠ, the amino acid sequence of this peptide was determined by N-terminal sequencing techniques. The amino-terminal half of the molecule was rich in charged amino acids and was hydrophilic, whereas the carboxyl-terminal half was hydrophobic.
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