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KMID : 0578319970070040572
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Molecules and Cells
1997 Volume.7 No. 4 p.572 ~ p.574
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Effect of Silkworm Hemolymph on the Expression of E. coli β-Galactosidase in Insect Cell Lines Infected with Recombinant Baculoviruses
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Woo, Soo Dong
Kim, Woo Jin/Kim, hey Seong/Choi, jae Young/Jin, Byung Rae/Kangm, Seok Kwon
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Abstract
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The effects of silkworm hemolymph on the expression of foregin genes by recombinant baculoviruses in cell lines were studied. The expression efficiency of β-galactosidase by recombinant virus containing the E. coli lacZ gene at various concentrations of hemolymph and FBS was determined in BmN and Sf cell lines. The addition of hemolymph to the medium containing FBS accelerated the expression of β-galactosidase by recombinant viruses in both cells. It was more effective in BmN cells than in Sf cells. Hemolymph was most effective in enhancing virus multiplicity under conditions of 5% FBS.
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