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KMID : 0578319970070050589
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Molecules and Cells
1997 Volume.7 No. 5 p.589 ~ p.593
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Transcription Effect of nm23-M2/NDP Kinase on c-myc Oncogene
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Lee, In hwan
chang, Sung Ik/Okada, kazuya/Bada, Hideo/Shiku, Hiroshi
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Abstract
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Transactivating factor PuF which interacts with a nuclease hypersensitive element locates upstream from the c-myc gene. PuF was recently identified as being encoded by nm23-H2/NDP kinase gene [Postel, E. H., Berberich, S.J., Flint, S. J., and Ferrone, C. A. (1993) Science 261, 428-429]. Here we have studed the correlation of expression between c-myc and nm23 genes in vitro. By a comparative study of the expression of 2 genes in cell lines, no direct correlation of kinetics was found. A plasmid containing the human c-myc fragment (c-myc CAT) was cloned upstream from the bacterial chloramphenicol acetyltransferase (CAT) gene. When the murine melanoma cell line was cotransfected with a nm23-M2/NDP kinase expression vector and c-myc CAT, CAT activity was elevated. In addition, cell cycle phases in the murine cell lines trasfected with nm23/NDP kinase were estimated; an alteration of the cell cycle, prolonged S-phase was found in the cell lines transfected with nm23-M2/NDP kinase, whereas human nm23-H2/NDP kinase did not play a role in transactivating the c-myc gene or in S-phase prolongation in murine cell lines. From these results we conclude that the murine nm23-M2 gene transactivates the c-myc gene and controls the cell cycle, S-phase, indirectly via a cellular cofactor in the murine cell line, which does not work with the human nm23-H2 gene.
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