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KMID : 0578319970070050641
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Molecules and Cells
1997 Volume.7 No. 5 p.641 ~ p.647
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An Alteration in the Structure of the MinorGroove of Duplex DNA Induced by the Formation of an Intermolecular d(GA)n
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Kim Moon-Gyo
R. Daniel Camerini-Otero
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Abstract
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A simple homopolymeric purine:purine·pyrimidine, d(GA)_(n):d(GA)_(n)´d(TC)_(n), triplex DNA was generated in the presence of magnesium ion at neutral pH. Hydrogen bonding of the N7 positions of guaniners and adenines was investigated by 7-deaza base substitutions and protection from chemical modification by dimethy sulfate (DMS). Chemical modification of this triplex with DMS revealed an unexpected triplex specific hypermodification in the N3 positions of the adenines in addition to the protection in the N7 positions of the guanines. The significance of this increase in the chemical accessibility of the N3 positions of adenines in the minor groove and possilbe relevance of dehydration to the mechanism of triplex formation and stability is discussed.
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