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KMID : 0578319970070050668
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Molecules and Cells
1997 Volume.7 No. 5 p.668 ~ p.673
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Isolation and Characterization of Cytosolic Copper/Zinc Superoxide Dismutase from Capsicum annuum L.
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Kim, Young Kyu
Kwon, soon Il/An, Chung sun
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Abstract
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A cDNA clone for a cytosolic Cu/Zn superoxide dismutase (Cu/ZnSOD) was isolated and characterized from red pepper (Capsicum annuum L.). The clone consisted of 735 hp containing one open reading frame (ORF) of 459 bp, 46 bp of 5´- and 230 bp of 3´-untranslated region. The nucleotide sequence of the ORF showed 93% homology with that of Nicotiana plumbaginifolia and tomato. It encodes a polypeptide of 154 amino acids with a molecular weight of 15,300. Genomic Southern hybridization suggested that only one copy is present. During cold treatment at 4 ℃, its expression was maintained at a similar level regardless of illumination.
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