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KMID : 0578319970070060783
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Molecules and Cells
1997 Volume.7 No. 6 p.783 ~ p.787
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Establishment of a Transgenic Tobacco Cell Suspension Culture system for producing Murine Granulocyte-Macrophage Colony Stimulating Factor
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Lee, Je-Sung
Choi, Seok-Jin/Kang, Hyun-Seok/Oh, Wha-Gyun/cho, Ki-haeng/Kwon, Tae-Ho/Kim, Dae-Hyuk
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Abstract
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We tested if murine granulocyte-macrophage colony stimulating factor (mGM-CSF) is produced as a biologically active form through plant cell culture. The mGM-CSF gene was cloned into the plant expresion vector, pBI121, and Ti-plasmid mediated trnasformation of tobacco leaves was conducted using Agrobacterium tumefaciens harboring the recombinant mGM-CSF (rmGM-CSF) gene. Cell suspension culture was established from the leaf-dervied calli of transgenic tabacco plants. Northern blot analysis indicated the expression of the introduced mGM-CSF gene in both transgenic plant and cell suspension cultures. In addition, the biological activity of rmGM-CSF from plant cell culture confirmed by measuring the proligeration of GM-CSF dependent FDC-P1 cells.
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