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KMID : 0578319970070060795
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Molecules and Cells
1997 Volume.7 No. 6 p.795 ~ p.799
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Promoter Region of the Drosophila melanogaster norpA Gene
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Doh, Seongwoong
Kim, Youn-Il/Yoon, Sangsoon/Lee, Seunghee/Baek, Kwanghee/Han, Kyuhyung/yoon, Jaeseung
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Abstract
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We have sequenced the 5´-flanking region of the Drosophila melanogaster norpA gene and characterized its promoter. The potential promoter region, which was deduced from the determination of the transcription start point (tsp), lacks a distinct TATA box sequence. Deletion analysis of the promoter reion suggests that the minimal promoter necessary for efficient expression of the gene is located between －138 (PstI) and ＋278 relativ to the tsp. Within this minimal promotor region, at least two downstream regulatory elements responsible for the stimulation of gene expression seem to exist in the DNA gragments between ＋44 and ＋121 and between ＋214 and ＋278. Among these, the DNA fragment between ＋44 and ＋121 affects promoter activity more dramatically (about 6-7 fold). This DNA fragment contains the consensus promoter element previously reported to be important for photoreceptor cell-specific expression, and this promoter element seems to be working in the norpA gene expression.
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