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KMID : 0578319990090010045
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Molecules and Cells
1999 Volume.9 No. 1 p.45 ~ p.48
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Expression, purification, characterization and crystallization of flap endonuclease-1 from Methanococcus jannaschii
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Bae KW
Baek KW/Cho CS/Hwang KY/Kim HR/Sung HC/Cho Y
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Abstract
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A gene coding for a protein homologous to the flap endonuclease-1 (FEN-1) was cloned from Methanococcus jannaschii, overexpressed, purified and characterized. The gene product from M. jannaschii shows 5’ endo-/exonuclease and 5’ pseudo-Y-endonuclease activities as observed in the FEN-1 in eukaryotes. In addition, Methanococcus jannaschii FEN-1 functions effectively at high concentrations of salt, unlike eukaryotic FEN-1. We have crystallized Methanococcus jannaschii FEN-1 and analyzed its preliminary character. The crystal belongs to the space group of P2(1) with unit cell dimensions of a = 58.93 A, b = 42.53 A, c = 62.62 A and beta = 92.250. A complete data set has been collected at 2.0 A resolution using a frozen crystal.
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KEYWORD
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