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KMID : 0578320000100010032
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Molecules and Cells
2000 Volume.10 No. 1 p.32 ~ p.37
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Dynamic Chromatin Remodeling in the Vicinity of J Chain Gene for the Regulation of Two Stage-specific Genes during B Cell Differentiation
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Kang Chang-Joong
Oh Uh-Taek
Marian Elliott Koshland
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Abstract
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Dynamic chromatin remodeling during B cell differentiation was identified in the vicinity of J chain gene. In pre-B cells, the enhancer-containing DNase I hypersensitive sites (HSSs) 3-4 were open. However, these HSSs 3-4 turned out to be unassociated with J chain gene expression, as the J chain promotercontaining HSSI remained in a closed state. The open
enhancer HSSs 3-4 in the pre-B cells might be related to the expression of a pre-B cell-specific gene upstream of the HSSs 3-4, which was identified in our Northern blot analyses. The HSSs 3-4 are then closed in the next immature and mature B cell stages until the IL-2 opens the HSSs 3-4 again as well as HSSI to express J chain gene in the primary immune responses. The dynamic regulation of chromatin structure during B cell differentiation for the expression of two stage-specific genes will provide a good model system for the study of B cell differentiation and gene expression.
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KEYWORD
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B Cell Differentiation, Chromatin, DNase Ⅰ Hypersensitive Site, J Chain Enhancer
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