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KMID : 0578320030150030301
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Molecules and Cells
2003 Volume.15 No. 3 p.301 ~ p.306
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Cloning and Expression of the Isoflavone Synthase Gene (IFS-Tp) from Trifolium paratense
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Kim, Bong Gyu
Kim, Song-Young/Song, Hee Su/Lee, Chan, Hur, Hor-Gil/Kim, Su-Il/Ahn, Joong-Hoon
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Abstract
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Isoflavones are secondary metabolites found mainly in leguminous plants. Their synthesis from flavanones is catalyzed by isoflavone synthase (IFS). We have cloned a isoflavone synthase gene (IFS-Tp) from Trifolium pratense that encodes a predicted 525 amino acids protein, molecular weight 59 kDa, with strong homology to IFS/s from other legumes. IFS-Tp was expressed in all the tissues examined, and addition of glutathione and UV irradiation enhanced its expression. Microsomes from yeast transformed with IFS-Tp were able to convert naringenin to genistein, indicating that IFS-Tp has isoflavone synthase activity.
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