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KMID : 0578320040170030430
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Molecules and Cells
2004 Volume.17 No. 3 p.430 ~ p.437
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Fibroblasts Enhance the In Vitro Survival of Human Memory and Naive B Cells by Maintaining Intracellular Levels of Glutathione
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Jeoung Euh-Jun
Lee In-Yong
Choi Jin-Suk
Cheon In-Su
Kang Gu
Choe Jong-Seon
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Abstract
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To gain insight into the mechanism of memory B cell survival, we cultured highly purified subpopulations of tonsillar B cells with tonsillar fibroblasts. The fibroblasts greatly enhanced the survival of memory and na?e B cells but did not delay the rapid apoptosis of germinal center B cells. B cell activation was not observed during the period of culture, as shown by the absence of activation markers and of cycling cells. These findings were reproduced when the B cells were physically separated from the fibroblasts by a semi-permeable transwell-membrane, indicating that the survival factor(s) were diffusible. Several cytokines including IL-6, IL-15, and VEGF were tested for survival activity but none could replace the fibroblasts. However, the addition of reduced glutathione (GSH) to the na?e and memory B cells significantly enhanced their survival, and depletion of GSH resulted in rapid loss of B cell viability. Furthermore, intracellular glutathione levels were maintained when the B cells were co-cultured with fibroblasts. Our results suggest that glutathione plays an important role in the survival of memory and na?e B cells in the presence of stromal cells.
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KEYWORD
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Fibroblast, Glutathione, Memory B Cell, Survial
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