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KMID : 0578320050200010083
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Molecules and Cells
2005 Volume.20 No. 1 p.83 ~ p.89
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HtrA2 Interacts with Aβ Peptide but Does Not Directly Alter Its Production or Degradation
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Liu ML
Liu MJ/Kim JM/Kim HJ/Kim JH/Hong ST
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Abstract
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HtrA2/Omi is a mammalian mitochondrial serine protease homologous to the E. coli HtrA/DegP gene products. Recently, HtrA2/Omi was found to have a dual role in mammalian cells, acting as an apoptosis-inducing protein and being involved in maintenance of mitochondrial homeostasis. By screening a human brain cDNA library with Ab peptide as bait in a yeast two-hybrid system, we identified HtrA2/Omi as a binding partner of Ab peptide. The interaction between Ab peptide and HtrA2/Omi was confirmed by an immunoblot binding assay. The possible involvement of HtrA2/Omi in Ab peptide metabolism was investigated. In vitro peptide cleavage assays showed that HtrA2/Omi did not directly promote the production of Ab peptide at the b/g-secretase level, or the degradation of Ab peptide. However, overexpression of HtrA2/Omi in K269 cells decreased the production of Ab40 and Ab42 by up to 30%. These results rule out the involvement of HtrA2/Omi in the etiology of Alzheimer’s disease. However, the fact that overexpression of HtrA2/Omi reduces the generation of Ab40 and Ab42 suggests that it may play some positive role in mammalian cells.
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KEYWORD
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